arabidopsis ath1 microarray gene chip  (Thermo Fisher)

Journal: Horticulture Research

Article Title: Diversity in global gene expression and morphology across a watercress ( Nasturtium officinale R. Br.) germplasm collection: first steps to breeding

doi: 10.1038/hortres.2015.29

Figure Lengend Snippet: (a) Transcriptomic differences between a novel dwarf watercress accession (dwarfAO) and the commercial (C) accession, where an up- or down- regulations, red and blue squares respectively is shown in dwarfAO compared to C. Mapman output illustrating down-regulation of genes in bins relating to plant growth in dwarfAO/C and an up-regulation of genes in the bins relating to plant defence in watercress accession dwarfAO/C. Superscript numbers denote Refs. , , , , , . (b) Overview of significantly different functional categories after microarray analysis. Colour scale represents fold changes, blue down-regulation and red up-regulation. Wilcoxon rank-sum test was applied to all three comparisons performed in PageMan. Clear visual representation of how the functional ontologies differ between the two watercress accessions.

Article Snippet: Variation in global gene expression was investigated using an Affymetrix Arabidopsis ATH1 microarray gene chip, using the commercial control (C), an accession selected for dwarf phenotype with a high AO potential (dwarfAO, called ‘Boldrewood’) and one with high AO potential alone.

Techniques: Functional Assay, Microarray

Journal: Nucleic Acids Research

Article Title: Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation

doi: 10.1093/nar/gkt296

Figure Lengend Snippet: Arabidopsis LSM orthologs

Article Snippet: To examine genome-wide mRNAs affected in the absence of AtLSM proteins, we analysed the expression and mRNA stability profiles in lsm1a lsm1b , lsm8 and sad1/lsm5 mutants using Affymetrix Arabidopsis ATH1 Gene-Chip microarrays.

Techniques:

Journal: Nucleic Acids Research

Article Title: Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation

doi: 10.1093/nar/gkt296

Figure Lengend Snippet: Expression of AtLSM genes in A. thaliana . Semi-quantitative RT-PCR analysis of AtLSM in leaves (Lv), stems (St), flowers (Fl), siliques (Sl) and roots (Rt). For AtLSM8 , both short and long mRNA versions are detected. M, DNA ladder. Elongation factor elF-4A was used as a control.

Article Snippet: To examine genome-wide mRNAs affected in the absence of AtLSM proteins, we analysed the expression and mRNA stability profiles in lsm1a lsm1b , lsm8 and sad1/lsm5 mutants using Affymetrix Arabidopsis ATH1 Gene-Chip microarrays.

Techniques: Expressing, Quantitative RT-PCR